To implement Single Sign-On (SSO) with JumpCloud, WorkOS, and Node.js, developers need to follow a step-by-step process. This includes installing the WorkOS Node SDK, setting up secrets, configuring the JumpCloud connection, setting up a redirect URI, and testing the connection. The SSO flow involves two main steps: initiating login and handling the callback. Developers can choose between using AuthKit for a customizable login box or building their own login box. Once the connection is set up, developers can test it using various scenarios provided by WorkOS. After successful integration, developers need to handle user sessions, implement logout, add social logins, implement access control, and more. The tutorial provides detailed instructions and resources for implementing SSO with JumpCloud, WorkOS, and Node.js.