The second day of Signal, a Twilio conference, saw attendees engage in various activities such as building with Lego kits, competing in coding challenges, and interacting with speakers including Harper Reed, Eric Ries, and They Might Be Giants. The event also marked the relaunch of TwilioFund, a $50 million investment fund focused on attracting new developers to Twilio's platform. Notable talks covered topics like one-click deployment features and WebRTC usage in businesses. The conference concluded with Jeff Lawson thanking attendees for their enthusiasm and ingenuity, marking the end of the first Signal event and opening up a new chapter for Twilio and its developer community.