Python continues to be a popular programming language, ranking third after Java and C, and consequently, the demand for Python-based test automation frameworks is growing. This text provides a comparative overview of five leading Python frameworks for test automation as of 2019: Robot Framework, Pytest, UnitTest (PyUnit), Behave, and Lettuce. Robot Framework is noted for its ease of use for beginners with its keyword-driven testing approach, while Pytest is praised for its compact and extensible test suites, albeit with compatibility limitations. UnitTest is recognized as a standard framework with minimal setup, though it requires extensive boilerplate code. Behave and Lettuce are highlighted for behavior-driven development, though they are primarily suitable for black-box testing. The author suggests Robot Framework for newcomers and emphasizes Pytest's superiority for functional testing, with pytest-bdd as a strong choice for those already familiar with Pytest for behavior-driven testing, aiming to guide users in selecting the most suitable framework for their needs.